Journal: eLife

Article Title: Chronic stress impairs autoinhibition in neurons of the locus coeruleus to increase asparagine endopeptidase activity

doi: 10.7554/eLife.106362

Figure Lengend Snippet: ( A, B ) Confocal images of LC neurons showing immunoreactivities for TH and α2A-ARs, together with a merged one in non-stress and 3-day RS mice. Arrowheads indicate the membrane regions of TH-positive neurons, along which α2A-ARs were differentially expressed between the control and the RS mice. Asterisks indicate LC neurons. ( C ) Western blotting analyses showing the expression of α2A-ARs in non-stress and 3-day RS mice ( n =6 and 7, respectively). Unpaired t-test, † p<0.001. ( D, E ) Confocal images of LC neurons in non-stress and RS mice. Upper panels from left to right showing the respective immunoreactivity for TH, α2A-ARs (with rabbit anti-α2A-AR) and Na + -K + -pump, and an enlarged image of the region enclosed with a rectangle (arrowheads) in its immediate left panel. Lower panels from left to right showing the merged image of TH and α2A-ARs, that of TH, α2A-ARs, and Na + -K + -pump, that of α2A-ARs and Na + -K + -pump, and an enlarged image of the region enclosed with a rectangle in its immediate left panel. ( F, G ) Western blotting analyses showing expressions of α2A-ARs and Na + -K + -pump in membrane fraction and those of α2A-ARs and β-actin in cytosol fraction in non-stress and 5-day RS mice ( n =3 and 3 samples, respectively). Each sample represents the analysis result in the LC tissues obtained from two to three mice. Membrane; unpaired t-test, † p=0.016. Cytosol; unpaired t-test, † p=0.083. Figure 3—source data 1. Original files for the western blot analysis in . Figure 3—source data 2. PDF containing the original blots in with the relevant bands clearly labeled. Figure 3—source data 3. Data used for graphs presented in .

Article Snippet: Antibody , Rabbit anti-α2A AR (polyclonal) , alomone , Cat# AAR-020, RRID: AB_10687546 , WB (1:500).

Techniques: Confocal, Membrane, Control, Western Blot, Expressing, Analysis, Labeling, Immunofluorescence

Journal: eLife

Article Title: Chronic stress impairs autoinhibition in neurons of the locus coeruleus to increase asparagine endopeptidase activity

doi: 10.7554/eLife.106362

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit anti-α2A AR (polyclonal) , alomone , Cat# AAR-020, RRID: AB_10687546 , WB (1:500).

Techniques:

Journal: BMC neuroscience

Article Title: Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α 2A -adrenergic receptor.

doi: 10.1186/s12868-024-00845-4

Figure Lengend Snippet: Fig. 4 Expression changes of ARs in rats exposed to noise and controls. a A schematic diagram illustrating the experimental procedure. b Western blot showing the expression changes of ARs in rats exposed to noise and controls (n = 6). c Quantification of the western blotting results. *p < 0.05, **p < 0.01. d Representative images showing DBH immunostaining at different positions along the base-to-apex axis. Rows and columns correspond to different positions along the cochlear axis, respectively, as indicated. Scale bar: 50 μm. e-f Representative images showing α2A-AR immunostaining at Organ of Corti and SGNs. Scale bar: 50 μm

Article Snippet: The blots were cut prior to hybridisation with antibodies during blotting, the membranes were incubated with specific primary antibodies: anti-α1A-specific polyclonal antibody (1:1000, 19777-1-AP, Proteintech), anti-α1B antibody (1:1000, DF8798, Affinity), anti-α1D antibody (0.2 μg/ml, ab166925, Abcam), anti-α2A polyclonal antibody (1:1000, 14266-1-AP, Proteintech), anti-α2B antibody (1:1000, A8535, ABclonal), anti-α2C antibody (1:1000, DF3108, Affinity), anti-β1 antibody (1:1000, bs-0498R, Bioss), anti-β2 antibody (1:1000, DF3512, Affinity), anti-β3 antibody (1:1000, bs-1063R, Bioss) and anti-GAPDH polyclonal antibody (1:10000, 10494-1-AP, Proteintech) overnight at 4 °C.

Techniques: Expressing, Western Blot, Immunostaining

Journal: BMC neuroscience

Article Title: Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α 2A -adrenergic receptor.

doi: 10.1186/s12868-024-00845-4

Figure Lengend Snippet: Fig. 5 Schematic diagram of potential protective mechanisms of NE against NIHL via α2A-AR. The levels of ROS increased after noise exposure in OHCs, leading to cell death and NIHL. Treatment with NE, an activator of α2A-AR, attenuated oxidative stress and protected from cell injury

Article Snippet: The blots were cut prior to hybridisation with antibodies during blotting, the membranes were incubated with specific primary antibodies: anti-α1A-specific polyclonal antibody (1:1000, 19777-1-AP, Proteintech), anti-α1B antibody (1:1000, DF8798, Affinity), anti-α1D antibody (0.2 μg/ml, ab166925, Abcam), anti-α2A polyclonal antibody (1:1000, 14266-1-AP, Proteintech), anti-α2B antibody (1:1000, A8535, ABclonal), anti-α2C antibody (1:1000, DF3108, Affinity), anti-β1 antibody (1:1000, bs-0498R, Bioss), anti-β2 antibody (1:1000, DF3512, Affinity), anti-β3 antibody (1:1000, bs-1063R, Bioss) and anti-GAPDH polyclonal antibody (1:10000, 10494-1-AP, Proteintech) overnight at 4 °C.

Techniques: